Comparative evaluation of the expression of enzymes of the ceramide <i>de novo</i> synthesis pathway in cardiac adipose tissue and blood vessels of cardiovascular patients

Aim . To compare the expression of enzymes ceramide de novo synthesis pathway in cardiac adipose tissue (AT) and blood vessels patients with coronary artery disease (CAD) acquired heart defects. Material methods The study included 20 CAD 18 aortic stenosis/regurgitation. Biopsies subcutaneous, epicardial, perivascular AT (SCAT, EAT, PVAT, respectively) were obtained during surgery. Quantitative PCR test was used to evaluate gene (serine palmitoyltransferase C1 C2: SPTLC1, SPTLC2; synthase 1-6: CERS1-6; dihydroceramide desaturase: DEGS1). Statistical analysis performed using GraphPad Prism 8 (GraphPad Software). Results. Patients characterized by a higher level mRNA SPTLC1 SCAT SPTLC2, CERS1, producing C18 ceramides, CERS5 CERS6, generating C14-C16 ceramides CERS2 — SCAT, long-chain C20-C24, CERS4, synthesizing very ceamides C18-C20. In high CERS4 CERS3, which synthesizes C26 higher, revealed. DEGS1 highest EAT. defects, there CERS3 EAT SPTLC2 all studied AT, 5 among than comparison group. Conclusion. Regional fat depots differed pathway. results indicate activation along this predominantly epicardial adipocytes pathology, may contribute accumulation localization.